Application

Preparation of Reagent
EZ-Float™ Tissue Mounting Reagent is provided as a 100X stock solution which must be diluted to a 1X working solution using PBS or deionized water immediately prior to use. The 1X working solution should be made fresh for each experiment.

EZ-Float™ Tissue Mounting Reagent is a novel aqueous solution for mounting free-floating tissue sections onto glass slides. EZ-Float™ enhances sample quality by minimizing folding or tearing of tissue sections, and reduces your sample processing time 3-4 fold compared to buffers such as PBS or TBS.

Disclaimer

For research and further manufacturing use only. It is not for use in humans.

General description

EZ-Float ™Tissue Mounting Reagent can be used to mount tissue sections to glass slides prior to antibody incubation and staining or after all incubation and staining steps are completed. Simply remove the buffer solution in which the tissue is suspended and replace it with freshly prepared 1X EZ-Float™ working solution. Then, using a large bore pipet, gently transfer the tissue and a small amount of reagent to a microscope slide. Alternatively, the tissue can be positioned on the slide by placing the glass slide directly in the 1X EZ-Float™ working solution and gently manipulating the tissue to the desired position on the glass. Finally, remove any excess solution by wicking off around the tissue.

EZ-Float™ Tissue Mounting Reagent is a novel aqueous solution made specifically for mounting free-floating tissue sections to glass microscope slides during immuno-histochemical (IHC) analysis. The EZ-Float™ Reagent enhances sample quality by minimizing folding or tearing of tissue sections. In addition, it facilitates the attachment of tissue to a variety of glass slides (coated and non-coated), which is often difficult when using buffered saline as the transfer solution. EZ-Float™ Reagent is also compatible with traditional IHC staining (like DAB) and even fluorescent detection. In conclusion, the EZ-Float™ Reagent dramatically reduces the sample processing time when compared to conventional tissue mounting procedures without impacting staining quality or intensity during IHC procedures.

Legal Information

EZ-FLOAT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage and Stability

Store the 100X EZ-Float™ Reagent at 2-8°C. When stored properly the 100X solution is stable for 12 months from date of receipt.